Phospho-slp-76 ser376

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August undefined, 2024

Webtion of SLP-76 at Ser376, but does not affect the level of total SLP-76 protein. The relationship between lysate protein concentration from untreated and H 2 O -treated Jurkat cells and the absorbance at 450 nm using the FastScan™ Phospho-SLP-76 (Ser376) ELISA Kit #30794 is shown in the upper figure. The corresponding western blots using Web76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 …

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Protocol for AlphaLISA SureFire Ultra SLP-76 (p …

WebSLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus … WebPE anti-SLP76 Phospho (Ser376) Download MSDS Product data sheet Product Type: Antibodies Single Brand: Sony Clone: A18001A Application: Intracellular Flow Cytometry Isotype: Mouse IgG1, κ Reactivity: … WebNo-wash kit for detection and quantitation of phospho-SLP-76 in cells using AlphaLISA SureFire technology. We are now two standalone organizations Life Sciences & … sinai land bridge

SLP-76 (Phospho-Ser376) TR-FRET Assay Kit - Cayman Chem

Technical Data Sheet

WebThis SLP-76 (Phospho-Ser376) TR-FRET Assay Kit uses a homogeneous TR-FRET assay method amenable to rapid measurement of phosphorylated protein levels in cells. This SLP-76 (Phospho-Ser376) TR-FRET Assay Kit is robust, with a Z’ value of 0.92, and suitable for screening a large number of samples. WebListed are ELISA Kits for the detection of SLP76, an alias name of lymphocyte cytosolic protein 2. The human protein, encoded by the gene LCP2, is 533 amino acid residues long and has a mass of 60,188 daltons. This protein is reported to have a cytoplasmic cellular localization. This target may also be known as: 76 kDa tyrosine phosphoprotein ... rcw title 82WebSLP-76 phosphorylated at Ser376 and another that recognizes an invariant epitope of SLP-76. ... Eu-Ab1 and FR-Ab2 (5 µL) for detection of phospho-SLP-76 (S376). • The plate was incubated at RT for . 18 hours. and the TR-FRET signal . was recorded at 665 and 615 nm (EnVision®; lamp excitation). sinai lifebridge health

"WebPhospho-SLP-76 (Ser376) (E3G9U) XP® Rabb. Catalog # Concentration Size List Price Quantity Your Price; 10190SF 100 µg: Add to Cart: Catalog # Size List Price Your Price; 10190SF 100 µg: Catalog # Qty: Add to Cart *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca " - Phospho-slp-76 ser376

Phospho-slp-76 ser376

WebPhospho-SLP-76 (Ser376) Antibody recognizes endogenous levels of SLP-76 protein only when phosphorylated at Ser376. This antibody cross-reacts with a 145 kDa protein of … WebAs Phospho SLP76 (Ser396) is a readout of T cell activation, it was used to monitor the inhibitory effect of PD-L1 recombinant protein on T cell activation. This assay was performed using the two-plate assay protocol. 400 000 Jurkat cells were plated in 25 µL of complete RPMI culture medium with 10% FBS.

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WebSLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T … WebJan 20, 2024 · We report here that SLP76-S376A-expressing mice exhibit normal thymocyte development and no detectable phenotypic alterations in mature T cell subsets or other lymphoid and myeloid cell lineages. Biochemical analyses revealed that mutant T cells were hypersensitive to TCR stimulation.

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WebMonoclonal Antibody for studying SLP76 (Ser376) phosphate. Validated for WB, IP, IHC, IHC, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SLP-76 … WebPhospho-SLP-76 (Tyr128) Fluorometric Cell-Based ELISA Kit Detection Target: SLP-76 Reactivity: Human, Mouse, Rat Quantity: 2x96 Wells Supplier Page Sign In or Register to …

Webendogenous SLP-76 at Ser376, in cellular lysates. The assay is an ideal system for the screening of both modulators of receptor activation (e.g. agonists and antagonists) as …

Web(14745) Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb Supplier Cell Signaling Technology. Host Rabbit Type Primary Clonality Recombinant (D9D6E) Recombinant Yes Conjugate Unconjugated Target Lymphocyte cytosolic protein 2 (Human) UniProt Q13094 - LCP2_HUMAN. Gene LCP2 Modification rcw title 80WebThis assay kit contains antibodies which recognize the phospho-Ser376 epitope, and a distal epitope, on SLP-76. The protein detected by this kit corresponds to GenBank Accession U20158.1. These antibodies recognize SLP-76 of human origin. Other species should be tested on a case-by-case basis. rcw title 9WebMouse phospho-SLP-76 (Ser376) control lysate HTRF® This lysate is a component of the mouse phospho-SLP-76 (Ser376) kit. It may be used as a residue specific positive control for mouse SLP-76 phosphorylation. See more Size Cat. number selected : - Quantity Request for quotation Prices may differ between countries. sinai house englandWebLymphocyte cytosolic protein (SLP-76) is required for T-cell activation and has been implicated in a number of signaling pathways. SLP76 is phosphorylated at Ser376 by HPK1, and this phosphorylation leads to a decrease in T-cell receptor signaling. o Description of the LANCE Ultra Assay sinai jewish primary school emailWebSLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. rcw tmv 2WebSLP-76 (Phospho-Ser376) Rabbit Polyclonal Antibody Product Code AMS.BT-AP04769-100ul Skip to the end of the images gallery Skip to the beginning of the images gallery $230.00 Qty Add to Cart Request Quote for Bulk Pricing Order Support T: +1 (617) 945 5033 T: +1 (800) 987 0985 (toll free) F: +1 (617) 945 8218 Email: [email protected] rcw tort liabilityWebThe Mouse Total SLP-76 cellular assay kit is designed to monitor the expression level of SLP-76, whether phosphorylated or unphosphorylated. It is compatible with our Mouse phospho-SLP-76 (Ser376) kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readouts of T-lymphocyte activation. Benefits rcw tolling