WebSamples were sonicated using a S220 focused-ultrasonicator (Covaris). Micro-Capture-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina. Libraries were amplified using 6 cycles on the thermocycler. Capture enrichment was done with IDT biotinylated DNA oligonucleotides and washes were performed using Roche … WebApr 8, 2024 · Find many great new & used options and get the best deals for COVARIS M220 FOCUSED ULTRASONICATOR TESTED TO POWERING ON at the best online …
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WebFigure 7. Highly reproducible final library size distribution is achieved with KAPA Pure Beads. All libraries were prepared with the KAPA HyperPrep Kit, from 1 μg high-quality E. coli genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator using conditions optimized for mode fragment lengths of 350 – 450 bp. Size selection (0.6X – … WebCovaris M220 Focused-ultrasonicator Watch on Specifications Features & Benefits Key Features Controlled energy delivery Isothermal processing Non-contact, closed vessel Quiet operation Robust and reliable design Tried and tested in major genome centers worldwide Benefits User-selectable fragment lengths No heat damage, high sample recovery how to download forge mod
Introducing the ML230 Focused-ultrasonicator - YouTube
WebMar 13, 2024 · Covaris LLC 14 Gill St, Unit H Woburn, Massachusetts 01801-1721 United States Phone: +1 781-932-3959 Fax: +1 781-932-8705 Email: [email protected] Company Profile Website: www.covaris.com Citations Target Product Catalog Vendor Reviews R230 Focused-ultrasonicator from Covaris LLC Get Quote WebApr 8, 2024 · Find many great new & used options and get the best deals for COVARIS M220 FOCUSED ULTRASONICATOR TESTED TO POWERING ON at the best online prices at eBay! Free shipping for many products! Web摘要: 以拟南芥成熟叶片为材料,探索3种不同型号超声破碎仪器的染色质免疫共沉淀(Ch IP)超声破碎条件.根据超声破碎结果推荐使用Covaris M220 Focused-ultrasonicator仪器,并将该仪器破碎条件设置为10%duty cycle,75 Watts Intensity Peak Incident power,200 cycle per Burst,7℃bath temperature,破碎时间12 min时,可获得约500 bp的DNA ... leather armor kidney belt