WebFIGURE 1. Cloning Efficiencies for Subcloned CHO-S and CHO-K1 Cells CHO-S (A) and CHO-K1 (B) cells were subcloned by limiting dilution in DMEM + 10% FBS, or ClonaCell™-CHO CD Liquid Medium containing either ClonaCell™-CHO ACF Supplement or ClonaCell™-CHO AOF Supplement; all conditions contained 6 - 8 mM L-glutamine. WebClonaCell™-CHO ACF is a methylcellulose-based semi-solid medium with supplements for the selection and cloning of suspension-adapted CHO cells in serum-free conditions. This medium is animal component-free and contains only recombinant proteins and synthetic components. Single-cell cloning efficiency of CHO cells is higher in this medium than ...
Sorting Single-Cell Suspension CHO Cells NanoCellect
Webof the ClonaCell™-CHO ACF Supplement significantly improved the cloning efficiencies in all four protein-free media to levels of 25 - 110% of that obtained in DMEM + 10% FBS. Serum-containing medium is often used to support the growth of CHO-S and CHO-K1 cells at low cell density. The use of FBS is undesirable, however, in many applications ... WebOct 11, 2012 · Serum-free and protein-free media (e.g., ClonaCell-CHO-ACF and ClonaCell-CHO-CD) are often preferred for cell line development, in particular for expression of recombinant proteins with potential therapeutic applications. Cells can be adapted slowly to a new medium by gradually increasing the relative volume of new … google chrome windows rt 8.1
ClonaCell™-CHO ACF Medium CHO Cell Selection Medium - STE…
Web03820 - ClonaCell™-CHO ACF Supplement Revision Number 2 Ingestion Clean mouth with water and drink afterwards plenty of water. Most important symptoms and effects, both acute and delayed Symptoms No information available. Indication of any immediate medical attention and special treatment needed Note to physicians Treat symptomatically. 5. WebClonaCell™-CHO ACF Supplement is a serum-free and animal component-free supplement that is added to protein-free media in order to increase cloning efficiency and promote robust growth of … Websupplement for cloning during CHO cell line development 03820 2.5 mL Product Description ClonaCell™-CHO ACF Supplement is a serum-free and animal component-free … google chrome windows / mac